发明申请
WO2012064381A1 STIMULI-RESPONSIVE POLYMER DIAGNOSTIC ASSAY COMPRISING MAGNETIC NANOPARTICLES AND CAPTURE CONJUGATES
审中-公开
基本信息:
- 专利标题： STIMULI-RESPONSIVE POLYMER DIAGNOSTIC ASSAY COMPRISING MAGNETIC NANOPARTICLES AND CAPTURE CONJUGATES
- 专利标题（中）：包含磁性纳米颗粒和捕获结合物的刺激性聚合物诊断测定
- 申请号：PCT/US2011/035256 申请日：2011-05-04
- 公开(公告)号：WO2012064381A1 公开(公告)日：2012-05-18
- 发明人： STAYTON, Patrick, S. , LAI, Jriuan , NEHILLA, Barrett, J. , SRINIVASAN, Selvi
- 申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION , STAYTON, Patrick, S. , LAI, Jriuan , NEHILLA, Barrett, J. , SRINIVASAN, Selvi
- 申请人地址： 4311 11th Avenue NE, Suite 500 Seattle, WA 98105-4608 US
- 专利权人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION,STAYTON, Patrick, S.,LAI, Jriuan,NEHILLA, Barrett, J.,SRINIVASAN, Selvi
- 当前专利权人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION,STAYTON, Patrick, S.,LAI, Jriuan,NEHILLA, Barrett, J.,SRINIVASAN, Selvi
- 当前专利权人地址： 4311 11th Avenue NE, Suite 500 Seattle, WA 98105-4608 US
- 代理机构： LAWSON, L., Rhys
- 优先权： US12/942,919 20101109
- 主分类号： G01N33/58
- IPC分类号： G01N33/58 ; G01N33/543 ; G01N33/553 ; B82Y25/00 ; C12N15/10
摘要：
The present disclosure utilizes the aggregation of stimuli-responsive polymers to isolate a diagnostic target (e.g., an antigen) from a solution using magnetophoresis. Isolating the diagnostic target provides a route to identify the presence of the diagnostic target in the solution. For example, utilized is a two-component assay (eg sandwich assay) comprising a magnetic nanoparticle functionalized with a stimuli-responsive polymer, and a capture molecule attached to a second stimuli-responsive polymer.
摘要（中）：
本公开使用刺激响应聚合物的聚集来使用磁电泳从溶液中分离诊断靶（例如抗原）。 隔离诊断目标提供了一种路径，用于识别解决方案中诊断目标的存在。 例如，所使用的是包含用刺激响应性聚合物官能化的磁性纳米颗粒和连接到第二刺激响应性聚合物的捕获分子的双组分测定（例如夹心测定）。