基本信息:
- 专利标题： ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS
- 专利标题（中）：用于植物基因工程的土地垫
- 申请号：PCT/US2011022145 申请日：2011-01-21
- 公开(公告)号：WO2011091317A3 公开(公告)日：2011-11-17
- 发明人： AINLEY WILLIAM MICHAEL , BLUE RYAN C , MURRAY MICHAEL G , CORBIN DAVID RICHARD , MILES REBECCA RUTH , WEBB STEVEN R
- 申请人： DOW AGROSCIENCES LLC , AINLEY WILLIAM MICHAEL , BLUE RYAN C , MURRAY MICHAEL G , CORBIN DAVID RICHARD , MILES REBECCA RUTH , WEBB STEVEN R
- 专利权人： DOW AGROSCIENCES LLC,AINLEY WILLIAM MICHAEL,BLUE RYAN C,MURRAY MICHAEL G,CORBIN DAVID RICHARD,MILES REBECCA RUTH,WEBB STEVEN R
- 当前专利权人： DOW AGROSCIENCES LLC,AINLEY WILLIAM MICHAEL,BLUE RYAN C,MURRAY MICHAEL G,CORBIN DAVID RICHARD,MILES REBECCA RUTH,WEBB STEVEN R
- 优先权： US29764110 2010-01-22
- 主分类号： A01H5/00
- IPC分类号： A01H5/00 ; C12N15/29 ; C12N15/82
摘要：
A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.
摘要（中）：
一种用于生产转基因植物的方法包括提供核酸分子，其包含与植物细胞的基因组DNA缺乏序列同源性的至少两个核酸序列区域和至少两个锌指核酸酶核酸酶识别位点，其中所述至少两个区域 与在至少两个锌指核酸酶识别位点相邻的植物细胞的基因组DNA缺乏序列同源性的核酸序列。 转化具有稳定整合到植物细胞基因组中的核酸分子的植物细胞或组织。 植物从植物细胞再生。 通过该方法产生转基因植物。 种子由转基因植物产生。
IPC结构图谱:
| A | 人类生活必需 |
| --A01 | 农业；林业；畜牧业；狩猎；诱捕；捕鱼 |
| ----A01H | 新植物或获得新植物的方法；通过组织培养技术的植物再生 |
| ------A01H5/00 | 有花植物，即被子植物 |