基本信息:
- 专利标题： CHIP FOR DETERMINATION OF MOLECULAR STRUCTURES AND FUNCTIONS
- 专利标题（中）：用于确定分子结构和功能的芯片
- 申请号：PCT/EP2010/004095 申请日：2010-07-07
- 公开(公告)号：WO2011003586A1 公开(公告)日：2011-01-13
- 发明人： DI BERARDINO, Marco , VOEROES, Janos , SUGIHARA, Kaori , ZAMBELLI, Tomaso
- 申请人： LEISTER PROCESS TECHNOLOGIES , DI BERARDINO, Marco , VOEROES, Janos , SUGIHARA, Kaori , ZAMBELLI, Tomaso
- 申请人地址： Galileostrasse 10 CH-6056 Kägiswil CH
- 专利权人： LEISTER PROCESS TECHNOLOGIES,DI BERARDINO, Marco,VOEROES, Janos,SUGIHARA, Kaori,ZAMBELLI, Tomaso
- 当前专利权人： LEISTER PROCESS TECHNOLOGIES,DI BERARDINO, Marco,VOEROES, Janos,SUGIHARA, Kaori,ZAMBELLI, Tomaso
- 当前专利权人地址： Galileostrasse 10 CH-6056 Kägiswil CH
- 代理机构： KLOCKE, Peter et al.
- 优先权： EP09008858.4 20090707
- 主分类号： G01N33/50
- IPC分类号： G01N33/50 ; G01N33/52 ; B01L3/00 ; G01N27/00 ; G01N33/487
摘要：
The present invention describes a chip for measuring transport properties of cell membranes or lipid bilayers comprising at least a substrate having a topside and a backside and plurality of nano- or micro-pores and a cell membrane covering the plurality of pores being accessible from both sides of the cell membrane for measurement. A material layer at least in the region of the pores to support the cell membrane at the pores but not hindering the transport through the cell membrane and the pores is arranged on the substrate, either on the topside or on the backside. Electrodes are attached to the substrate for electrochemically measurement of the differences in the current through the membrane and the pores for monitoring transport processes. Both sides of the membrane are accessible by this structure.
摘要（中）：
本发明描述了一种用于测量细胞膜或脂质双层的输送性能的芯片，其包括至少具有顶侧和背面以及多个纳米或微孔的基底和覆盖多个孔的细胞膜，两个孔可从两侧接近 的细胞膜用于测量。 至少在孔的区域中的材料层，以在孔处支撑细胞膜但不阻碍细胞膜的传输，并且孔在顶侧或背面上布置在基底上。 电极连接到基底上，用于电化学测量通过膜和孔的电流差异，用于监测运输过程。 膜的两侧可通过该结构进入。