基本信息:
- 专利标题： CONJUGATES OF BIOMOLECULES TO NANOPARTICLES
- 专利标题（中）：生物分子与纳米颗粒的缀合物
- 申请号：PCT/US2010028974 申请日：2010-03-26
- 公开(公告)号：WO2010111691A3 公开(公告)日：2011-03-31
- 发明人： NIKIFOROV THEO , MAZUR DANIEL , PEDG XINZHAN , LINCECUM TOMMIE , BELOSLUDTSEV YURI , REESE HOWARD , GREMYACHINSKIY DMITRIY , ROZHKOV ROMAN , MAURO JOHN , BEECHEM JOSEPH , TULSKY ERIC , NAASANI IMAD , HALEY KARI , TREADWAY JOSEPH
- 申请人： LIFE TECHNOLOGIES CORP , NIKIFOROV THEO , MAZUR DANIEL , PEDG XINZHAN , LINCECUM TOMMIE , BELOSLUDTSEV YURI , REESE HOWARD , GREMYACHINSKIY DMITRIY , ROZHKOV ROMAN , MAURO JOHN , BEECHEM JOSEPH , TULSKY ERIC , NAASANI IMAD , HALEY KARI , TREADWAY JOSEPH
- 专利权人： LIFE TECHNOLOGIES CORP,NIKIFOROV THEO,MAZUR DANIEL,PEDG XINZHAN,LINCECUM TOMMIE,BELOSLUDTSEV YURI,REESE HOWARD,GREMYACHINSKIY DMITRIY,ROZHKOV ROMAN,MAURO JOHN,BEECHEM JOSEPH,TULSKY ERIC,NAASANI IMAD,HALEY KARI,TREADWAY JOSEPH
- 当前专利权人： LIFE TECHNOLOGIES CORP,NIKIFOROV THEO,MAZUR DANIEL,PEDG XINZHAN,LINCECUM TOMMIE,BELOSLUDTSEV YURI,REESE HOWARD,GREMYACHINSKIY DMITRIY,ROZHKOV ROMAN,MAURO JOHN,BEECHEM JOSEPH,TULSKY ERIC,NAASANI IMAD,HALEY KARI,TREADWAY JOSEPH
- 优先权： US16432409 2009-03-27; US29361610 2010-01-08; US29991910 2010-01-29; US30735610 2010-02-23; US29361810 2010-01-08; US26397409 2009-11-24; US24545709 2009-09-24; US24277109 2009-09-15; US29991710 2010-01-29; US18477009 2009-06-05; US28938809 2009-12-22
- 主分类号： C12Q1/48
- IPC分类号： C12Q1/48 ; C12Q1/68 ; G01N33/52 ; G01N33/573
摘要：
Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule: nanoparticle ratio approaches or equals 1 :1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.
摘要（中）：
本文公开了包含与标签连接的生物分子的缀合物，所述生物分子具有生物活性并且可用于各种生物应用。 例如，本文提供了聚合酶 - 纳米颗粒缀合物，其包括连接至纳米颗粒的聚合酶，其中缀合物具有聚合酶活性。 这种缀合物可表现出降低的聚集和改进的化学计量学，其中平均生物分子：纳米颗粒比例接近或等于1：1。 本文还公开了用于制备这种缀合物的改进方法，以及在生物应用中使用这种缀合物的方法和系统，所述生物应用例如核苷酸掺入，引物延伸和单分子测序。
公开/授权文献:
- WO2010111691A9 CONJUGATES OF BIOMOLECULES TO NANOPARTICLES 公开/授权日：2011-01-06