基本信息:
- 专利标题: METHOD OF BIOSYNTHESIZING TETRODOTOXIN
- 专利标题(中):四羟甲基纤维素生物合成方法
- 申请号:PCT/IB2008000408 申请日:2008-02-25
- 公开(公告)号:WO2008102253A3 公开(公告)日:2008-11-20
- 发明人: YU HOI-FU PETER , YU FAI CHUN
- 申请人: UNIV HONG KONG POLYTECHNIC , YU HOI-FU PETER , YU FAI CHUN
- 专利权人: UNIV HONG KONG POLYTECHNIC,YU HOI-FU PETER,YU FAI CHUN
- 当前专利权人: UNIV HONG KONG POLYTECHNIC,YU HOI-FU PETER,YU FAI CHUN
- 优先权: US70965707 2007-02-23
- 主分类号: C12P17/18
- IPC分类号: C12P17/18 ; C12N1/20 ; C12R1/64
摘要:
Provided are methods for the biosynthesis of tetrodotoxin (TTX) comprising the steps of: obtaining a culture from a seed culture or storage possessing one or more of Vibrio species, inoculating the culture and a growth tissue extract from a tetrodotoxin-bearing organism in a fermentation medium, and isolating and purifying TTX from the said fermentation medium, resulting in a yield of at least 90% pure TTX of about 0.5 mg TTX/L in a maximum of 3 to 5 days.
摘要(中):
提供了用于生物合成河豚毒素(TTX)的方法,包括以下步骤:从具有一种或多种弧菌种的种子培养物或储存器获得培养物,在发酵中接种培养物和来自含河豚毒素的生物的生长组织提取物 培养,并从所述发酵培养基中分离和纯化TTX,得到最多3至5天的至少90%纯TTX的产量为约0.5mg TTX / L。