发明申请
WO2008097719A1 HEMOLYTIC REAGENT AND METHOD FOR THE STUDY OF LEUKOCYTE SUBPOPULATIONS IN WHOLE BLOOD
审中-公开
基本信息:
- 专利标题： HEMOLYTIC REAGENT AND METHOD FOR THE STUDY OF LEUKOCYTE SUBPOPULATIONS IN WHOLE BLOOD
- 专利标题（中）：全血白细胞生长因子研究的化学试剂及方法
- 申请号：PCT/US2008/051855 申请日：2008-01-24
- 公开(公告)号：WO2008097719A1 公开(公告)日：2008-08-14
- 发明人： DEKA, Chiranjit , FENG, Jianwen
- 申请人： IDEXX LABORATORIES, INC. , DEKA, Chiranjit , FENG, Jianwen
- 申请人地址： One Idexx Drive Westbrook, ME 04092 US
- 专利权人： IDEXX LABORATORIES, INC.,DEKA, Chiranjit,FENG, Jianwen
- 当前专利权人： IDEXX LABORATORIES, INC.,DEKA, Chiranjit,FENG, Jianwen
- 当前专利权人地址： One Idexx Drive Westbrook, ME 04092 US
- 代理机构： CAMPAGNA, Pina, M. et al.
- 优先权： US11/626,759 20070124
- 主分类号： G01N33/50
- IPC分类号： G01N33/50
摘要：
A lytic reagent composition and methods for differential analysis of leukocytes are disclosed. In embodiments, the analysis may utilize optical measurements in flow cytometry based hematology analyzers. The reagent system includes an anionic surfactant in a hypotonic solution, an inorganic buffer to maintain the pH in a range from about 6 to about 10, and optionally a leukocyte stabilizer. The reagent system is used to lyse red blood cells and stabilize the leukocytes to enable multi-part differentiation of leukocytes in a near physiologic pH environment on a flow cytometry based hematology analyzer using axial light loss, light scatter intensity, high-numerical aperture side scatter, and time-of-flight measurements.
摘要（中）：
公开了一种裂解试剂组合物和白细胞差异分析方法。 在实施例中，分析可以利用基于流式细胞术的血液分析仪中的光学测量。 试剂系统包括在低渗溶液中的阴离子表面活性剂，将pH保持在约6至约10范围内的无机缓冲剂和任选的白细胞稳定剂。 试剂系统用于裂解红血细胞并稳定白细胞，使得在基于流式细胞仪的血液分析仪上，在近生理pH环境中白细胞多部分分化，使用轴向光损失，光散射强度，高数值孔径侧散射 和飞行时间测量。