基本信息:
- 专利标题: HIGH-THROUGHPUT AUTOMATED CELLULAR INJECTION SYSTEM AND METHOD
- 专利标题(中):高通自动化细胞注射系统及方法
- 申请号:PCT/CA2007/001685 申请日:2007-09-21
- 公开(公告)号:WO2008034249A1 公开(公告)日:2008-03-27
- 发明人: SUN, Yu , WANG, Wenhui , LIU, Xinyu
- 申请人: SUN, Yu , WANG, Wenhui , LIU, Xinyu
- 申请人地址: 909 Bay Street, Suite 2607 Toronto, Ontario M5S 3G2 CA
- 专利权人: SUN, Yu,WANG, Wenhui,LIU, Xinyu
- 当前专利权人: SUN, Yu,WANG, Wenhui,LIU, Xinyu
- 当前专利权人地址: 909 Bay Street, Suite 2607 Toronto, Ontario M5S 3G2 CA
- 代理机构: DE FAZEKAS, Anthony
- 优先权: CA2,560,352 20060921
- 主分类号: C12M3/00
- IPC分类号: C12M3/00 ; B01L3/02 ; C12M1/00 ; C12N15/89 ; G01N35/10
摘要:
An automated cell injection system and method are described, which can perform automatic, reliable, and high- throughput cell injection of foreign genetic materials, proteins, and other compounds. The system and method overcome the problems inherent in traditional manual injection that is characterized by poor reproducibility, human fatigue, and low throughput. The present invention is particularly suited for zebrafish embryo injection but can be readily extended to other biological injection applications such as mouse embryo, drosophila embryo, and C. elegans injections, capable of facilitating high-throughput genetic research at both academic and industry levels. A novel vacuum based cell-holding device is also provided.
摘要(中):
描述了自动细胞注射系统和方法,其可以执行外来遗传物质,蛋白质和其它化合物的自动,可靠和高通量的细胞注射。 该系统和方法克服了传统手动注射固有的问题,其特点是重现性差,人体疲劳和生产能力低。 本发明特别适用于斑马鱼胚胎注射,但可以容易地扩展到其他生物注射应用例如小鼠胚胎,果蝇胚胎和秀丽隐杆线虫注射液,能够促进学术和工业水平的高通量遗传研究。 还提供了一种新颖的基于真空的电池保持装置。
IPC结构图谱:
C | 化学;冶金 |
--C12 | 生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程 |
----C12M | 酶学或微生物学装置 |
------C12M3/00 | 组织、人类、动物或植物细胞或病毒培养装置 |