基本信息:
- 专利标题: METHOD FOR THE EXTRACTION OF ONE OR SEVERAL PROTEINS PRESENT IN MILK
- 专利标题(中):提取一种或几种蛋白质的方法
- 申请号:PCT/FR2007000908 申请日:2007-05-31
- 公开(公告)号:WO2007138198A3 公开(公告)日:2009-04-23
- 发明人: LEJARS ALAIN , NOGRE MICHEL , TELLIER MICHEL
- 申请人: LFB BIOTECHNOLOGIES , LEJARS ALAIN , NOGRE MICHEL , TELLIER MICHEL
- 专利权人: LFB BIOTECHNOLOGIES,LEJARS ALAIN,NOGRE MICHEL,TELLIER MICHEL
- 当前专利权人: LFB BIOTECHNOLOGIES,LEJARS ALAIN,NOGRE MICHEL,TELLIER MICHEL
- 优先权: FR0604864 2006-05-31
- 主分类号: C07K1/14
- IPC分类号: C07K1/14 ; C12N15/85
摘要:
This invention concerns a method for extracting at least one protein present in milk, the said protein showing an affinity for calcium ions in complexes or free, in the said milk. It comprises the following steps: a) freeing the protein by precipitating the calcium compounds obtained by contact of the milk with a soluble salt, whose anion is chosen for its capacity to form the said insoluble calcium compounds in such a medium, in order to thus obtain a liquid phases that is enriched with the protein, b) separation of the enriched liquid phase into the protein precipitate with the calcium compound, the said liquid phase being moreover separated into a lipid phase and a non-lipid aqueous phase comprising the protein and c) collection of the aqueous non-lipid phase comprising the protein.
摘要(中):
本发明涉及一种提取乳中存在的至少一种蛋白质的方法,所述蛋白质表现出对所述牛奶中复合物中钙离子或游离的亲和力。 它包括以下步骤:a)通过沉淀由乳与可溶性盐接触获得的钙化合物来释放蛋白质,其中阴离子被选择用于在这种介质中形成所述不溶性钙化合物,从而 获得富含蛋白质的液相,b)用钙化合物将富集的液相分离成蛋白沉淀物,此外将所述液相分离成脂质相和包含蛋白质的非脂质水相, c)收集包含该蛋白质的非水相含水相。