发明申请
WO2007068265A1 HYBRID 3’ UNTRANSLATED REGIONS SUITABLE FOR EFFICIENT PROTEIN EXPRESSION IN MAMMALIAN CELLS
审中-公开
基本信息:
- 专利标题： HYBRID 3’ UNTRANSLATED REGIONS SUITABLE FOR EFFICIENT PROTEIN EXPRESSION IN MAMMALIAN CELLS
- 专利标题（中）：混合3'适用于马马里兰细胞中有效蛋白表达的不分割区域
- 申请号：PCT/EP2005/013316 申请日：2005-12-12
- 公开(公告)号：WO2007068265A1 公开(公告)日：2007-06-21
- 发明人： ABU KHABAR, Khalid, S.
- 申请人： KING FAISAL SPECIALIST HOSPITAL & RESEARCH CENTRE , TERRAMARK MARKENCREATION GMBH , ABU KHABAR, Khalid, S.
- 申请人地址： Takhasusi Street, P.O. Box 3354, MBC-03-067, Riyadh 11211 SA
- 专利权人： KING FAISAL SPECIALIST HOSPITAL & RESEARCH CENTRE,TERRAMARK MARKENCREATION GMBH,ABU KHABAR, Khalid, S.
- 当前专利权人： KING FAISAL SPECIALIST HOSPITAL & RESEARCH CENTRE,TERRAMARK MARKENCREATION GMBH,ABU KHABAR, Khalid, S.
- 当前专利权人地址： Takhasusi Street, P.O. Box 3354, MBC-03-067, Riyadh 11211 SA
- 代理机构： ENGELHARD, Markus et al.
- 主分类号： C12N15/85
- IPC分类号： C12N15/85
摘要：
The present invention describes the use of hybrid short 3’ untranslated (3’UTR) regions which are composed of two regions, one region from an 3’ untranslated region of a stable eukaryotic mRNA, and another region from the downstream end of an 3’ untranslated region of another eukaryotic mRNA that contains a polyadenylation (polyA) signal. The use of such hybrid regions allows for an efficient protein expression when used in conjunction with circular or linear expression DNA molecules. The present invention provides a recombinant DNA that is composed of a promoter, a protein coding region, and the hybrid 3’UTR in a continuous and directional orientation. The efficient expression systems of the invention are suitable for the economical and efficient production of therapeutic proteins and for use with both transient and stable expression systems.
摘要（中）：
本发明描述了由两个区域组成的杂交短3'非翻译区（3'UTR）的区域，来自稳定真核mRNA的3'非翻译区的一个区域和3'末端的另一个区域 含有多聚腺苷酸化（polyA）信号的另一个真核mRNA的非翻译区。 当与环状或线性表达DNA分子结合使用时，这种杂交区域的使用允许有效的蛋白质表达。 本发明提供由连续和定向取向的启动子，蛋白质编码区和杂合3'UTR组成的重组DNA。 本发明的有效表达系统适用于治疗性蛋白质的经济和有效的生产，并且与瞬时和稳定的表达系统一起使用。