基本信息:
- 专利标题： DNA FINGERPRINTING USING A BRANCH MIGRATION ASSAY
- 专利标题（中）：使用分支迁移测定的DNA指纹印记
- 申请号：PCT/US2005/033838 申请日：2005-09-21
- 公开(公告)号：WO2006034349A2 公开(公告)日：2006-03-30
- 发明人： POURMAND, Nader , DAVIS, Ronald, W. , WANG, Shan, X.
- 申请人： THE BOARD OF TRUSTEES OF THE LELAND STANDFORD JUNIOR UNIVERSITY , POURMAND, Nader , DAVIS, Ronald, W. , WANG, Shan, X.
- 申请人地址： Stanford University, Office of Technology Licensing, 1705 El camino Real, Palo Alto, CA 94306 US
- 专利权人： THE BOARD OF TRUSTEES OF THE LELAND STANDFORD JUNIOR UNIVERSITY,POURMAND, Nader,DAVIS, Ronald, W.,WANG, Shan, X.
- 当前专利权人： THE BOARD OF TRUSTEES OF THE LELAND STANDFORD JUNIOR UNIVERSITY,POURMAND, Nader,DAVIS, Ronald, W.,WANG, Shan, X.
- 当前专利权人地址： Stanford University, Office of Technology Licensing, 1705 El camino Real, Palo Alto, CA 94306 US
- 代理机构： LUMEN IPS
- 优先权： US60/612,000 20040921
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
A method of determining the length of a polynucleotide target is provided. With this method, a target is first hybridized to an array of first probes having different, determined lengths, resulting in the formation of duplexes between the polynucleotide target and the first probes. These duplexes have a single stranded section of target if the target is longer than the first probe it is in a duplex with. Next, a second probe having a determined length is hybridized to these duplexes. If the length of the target is greater than the length of the first probe it is displaced during this hybridization step by the process of branch migration. In contrast, if the length of the target is less than or equal to the length of the first probe, it is not displaced. Thus, the length of the polynucleotide target can be determined.
摘要（中）：
提供了确定多核苷酸靶标长度的方法。 利用该方法，首先将靶标与具有不同确定长度的第一探针阵列杂交，导致多核苷酸靶标与第一探针之间形成双链体。 如果目标长度大于第一个探针与双工目标的距离，则这些双链具有单链目标部分。 接下来，具有确定长度的第二探针与这些双链体杂交。 如果目标的长度大于第一个探针的长度，则在该杂交步骤期间通过分支迁移的过程而被移位。 相反，如果目标的长度小于或等于第一个探针的长度，则不会发生位移。 因此，可以确定多核苷酸靶标的长度。