基本信息:
- 专利标题： IN VITRO TRANSLATION SYSTEM
- 申请号：PCT/US2004/013110 申请日：2004-04-27
- 公开(公告)号：WO2004096999A3 公开(公告)日：2004-11-11
- 发明人： LEE, Jae, Moon , BUCKLEY, Douglas, Iwen , CANCILLA, Michael, Robert , CURTIS, Damian, E. , BOWMAN, Krista, K. , ZHAN, Hangjun , CIANCIO, Margie
- 申请人： EXELIXIS, INC. , LEE, Jae, Moon , BUCKLEY, Douglas, Iwen , CANCILLA, Michael, Robert , CURTIS, Damian, E. , BOWMAN, Krista, K. , ZHAN, Hangjun , CIANCIO, Margie
- 申请人地址： P.O. Box 511, 170 Harbor Way, South San Francisco, CA 94083-0511 US
- 专利权人： EXELIXIS, INC.,LEE, Jae, Moon,BUCKLEY, Douglas, Iwen,CANCILLA, Michael, Robert,CURTIS, Damian, E.,BOWMAN, Krista, K.,ZHAN, Hangjun,CIANCIO, Margie
- 当前专利权人： EXELIXIS, INC.,LEE, Jae, Moon,BUCKLEY, Douglas, Iwen,CANCILLA, Michael, Robert,CURTIS, Damian, E.,BOWMAN, Krista, K.,ZHAN, Hangjun,CIANCIO, Margie
- 当前专利权人地址： P.O. Box 511, 170 Harbor Way, South San Francisco, CA 94083-0511 US
- 代理机构： SHAYESTEH, Laleh
- 优先权： US60/465,963 20030428
- 主分类号： C12P21/00
- IPC分类号： C12P21/00
摘要：
In vitro translation (IVT) systems and methods for increased expression of proteins from linear templates, using GamS, are provided. The proteins may be full length or protein fragments. The IVT system may be used in batch or continuous mode. The GamS may be used as GamS nucleic acid template, crude protein fraction, or purified protein product. The IVT system using GamS component may be employed in a high-throughput mode. The ability to predict expressible protein or fragments, and activity and solubility of a large-scale protein expression product based on the results obtained from high-throughput, small-scale IVT expression product is also provided.
IPC结构图谱:
| C | 化学；冶金 |
| --C12 | 生物化学；啤酒；烈性酒；果汁酒；醋；微生物学；酶学；突变或遗传工程 |
| ----C12P | 发酵或使用酶的方法合成目标化合物或组合物或从外消旋混合物中分离旋光异构体 |
| ------C12P21/00 | 肽或蛋白质的制备 |