基本信息:
- 专利标题： A SYSTEM AND METHOD FOR AUTOMATIC PROTEIN SEQUENCING BY MASS SPECTROMETRY
- 专利标题（中）：一种通过质谱分析自动蛋白质测序的系统和方法
- 申请号：PCT/EP2001/014041 申请日：2001-11-30
- 公开(公告)号：WO2003046577A1 公开(公告)日：2003-06-05
- 发明人： WILM, Matthias , NEUBAUER, Gitte, Jackie
- 申请人： THE EUROPEAN MOLECULAR BIOLOGY LABORATORY , WILM, Matthias , NEUBAUER, Gitte, Jackie
- 申请人地址： Meyerhofstrasse 1, 69117 Heidelberg DE
- 专利权人： THE EUROPEAN MOLECULAR BIOLOGY LABORATORY,WILM, Matthias,NEUBAUER, Gitte, Jackie
- 当前专利权人： THE EUROPEAN MOLECULAR BIOLOGY LABORATORY,WILM, Matthias,NEUBAUER, Gitte, Jackie
- 当前专利权人地址： Meyerhofstrasse 1, 69117 Heidelberg DE
- 代理机构： SILVESTON, Judith
- 主分类号： G01N33/68
- IPC分类号： G01N33/68
摘要：
A method of deducing the sequence of a protein by analysing tandem mass spectrometry data. The protein is subjected to partial isotopic labelling by enzymatic digestion in a water mixture comprising a non-natural abundance of H 2 1s O. Differential scanning mass spectrometry is applied to the peptide fragments obtained from the digestion. Peaks in the spectra are analyzed to ascertain whether they arise from isotopically labeled fragments or not. A filtered spectrum is calculated that just comprises peaks from the y-ions. The sequence of the peptide is deduced by computing the difference in the mass between adjacent y-ion peaks.
摘要（中）：
通过分析串联质谱数据推导蛋白质序列的方法。 蛋白质通过在包含非天然丰度的H2 <1s> O的水混合物中通过酶消化进行部分同位素标记。 差示扫描质谱法应用于从消化获得的肽片段。 分析光谱中的峰，以确定它们是否来自同位素标记的片段。 计算出仅包含来自y离子的峰的滤波光谱。 通过计算相邻y离子峰之间的质量差异来推导肽的序列。