基本信息:
- 专利标题： UNCOUPLED PRODUCTIVE AND CATABOLIC HOST CELL PATHWAYS
- 专利标题（中）：未经协调的生产性和可驱动的宿主细胞途径
- 申请号：PCT/US0210575 申请日：2002-04-04
- 公开(公告)号：WO02081631A3 公开(公告)日：2002-11-28
- 发明人： DODGE TIMOTHY C , VALLE FERNANDO
- 申请人： GENENCOR INT , DODGE TIMOTHY C , VALLE FERNANDO
- 专利权人： GENENCOR INT,DODGE TIMOTHY C,VALLE FERNANDO
- 当前专利权人： GENENCOR INT,DODGE TIMOTHY C,VALLE FERNANDO
- 优先权： US28161801 2001-04-04; US28225901 2001-04-05
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12N1/21 ; C12N9/12 ; C12N15/52 ; C12P7/60 ; C12P19/02 ; C12P19/30 ; C12P25/00 ; C12R1/01 ; C12N9/00 ; C07H21/04 ; C12N1/20
摘要：
The invention provides methods for producing products comprising improved host cells genetically engineered to have uncoupled productive and catabolic pathways. In particular, the present invention provides host cells having a modification in nucleic acid encoding an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a modification of nucleic acid encoding an enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such improved host cells are used for the production of products, such as, ascorbic acid intermediates. Methods for making and using the improved host cells are provided. Nucleic acid and amino acid sequences for glucokinase and gluconokinase are provided.
摘要（中）：
本发明提供了用于生产产品的方法，所述产品包含基因工程化以具有未偶联的生产和分解代谢途径的改良的宿主细胞。 具体而言，本发明提供了在编码内源酶活性的核酸中具有修饰的宿主细胞，所述内源酶活性在其第6个碳处磷酸化D-葡萄糖和/或编码在其第6个碳处磷酸化D-葡萄糖酸的酶活性的核酸的修饰 。 这种改进的宿主细胞用于生产产品，例如抗坏血酸中间体。 提供了制备和使用改进的宿主细胞的方法。 提供了葡萄糖激酶和葡萄糖激酶的核酸和氨基酸序列。