基本信息:
- 专利标题： APTAMERS SPECIFIC FOR THROMBIN AND METHODS OF USE
- 专利标题（中）：适用于沙门氏菌的APTAMERS及其使用方法
- 申请号：PCT/US1992001367 申请日：1992-02-21
- 公开(公告)号：WO1992014842A1 公开(公告)日：1992-09-03
- 发明人： GILEAD SCIENCES, INC.
- 申请人： GILEAD SCIENCES, INC. , TOOLE, John, J. , GRIFFIN, Linda, C. , BOCK, Louis, C. , LATHAM, John, A. , KRAWCZYK, Steven
- 专利权人： GILEAD SCIENCES, INC.,TOOLE, John, J.,GRIFFIN, Linda, C.,BOCK, Louis, C.,LATHAM, John, A.,KRAWCZYK, Steven
- 当前专利权人： GILEAD SCIENCES, INC.,TOOLE, John, J.,GRIFFIN, Linda, C.,BOCK, Louis, C.,LATHAM, John, A.,KRAWCZYK, Steven
- 优先权： US658,849 19910221; US659,981 19910221; US745,215 19910814; US744,870 19910814; US787,921 19911106
- 主分类号： C12Q01/68
- IPC分类号： C12Q01/68
摘要：
Oligonucleotide sequences that mediate specific binding to thrombin and optionally contain modified bases, sugars, or sugar linkages are disclosed. Single-stranded DNA oligomers are obtained that bind thrombin and inhibit its function in vitro and in vivo. The thrombin binding oligomers are useful for therapeutic, diagnostic and manufacturing purposes. An improved method for identifying these oligomers is also described, involving complexation of the support-bound thrombin with a mixture of oligonucleotides containing random sequences under conditions wherein a complex is formed with the specifically binding sequences, but not with the other members of the oligonucleotide mixture. The thrombin-oligonucleotide complexes are then separated from both the support and the uncomplexed oligonucleotides and the complexed members of the oligonucleotide mixture are recovered from the separated complex and subsequently amplified using standard techniques.
摘要（中）：
公开介导特异性结合凝血酶并任选地含有修饰的碱基，糖或糖键的寡核苷酸序列。 获得结合凝血酶并在体外和体内抑制其功能的单链DNA寡聚体。 凝血酶结合寡聚体可用于治疗，诊断和制造目的。 还描述了用于鉴定这些寡聚体的改进方法，包括在其中使用特异性结合序列而不与寡核苷酸混合物的其它成员形成复合物的条件下使载体结合的凝血酶与含有随机序列的寡核苷酸的混合物络合 。 然后从载体和未复合的寡核苷酸中分离出凝血酶 - 寡核苷酸复合物，并从分离的复合物中回收寡核苷酸混合物的复合成员，随后使用标准技术扩增。