发明申请
US20220282294A1 Primer, Device for Producing Double-Stranded DNA Using Primer, and Method for Producing Double-Stranded DNA Using Primer
有权
基本信息:
- 专利标题： Primer, Device for Producing Double-Stranded DNA Using Primer, and Method for Producing Double-Stranded DNA Using Primer
- 申请号：US17631407 申请日：2020-07-31
- 公开(公告)号：US20220282294A1 公开(公告)日：2022-09-08
- 发明人： Hiroshi Abe , Naoko Abe , Kosuke Nakamoto , Hiroki Murase , Yasuaki Kimura
- 申请人： Japan Science and Technology Agency
- 申请人地址： JP Kawaguchi-shi
- 专利权人： Japan Science and Technology Agency
- 当前专利权人： Japan Science and Technology Agency
- 当前专利权人地址： JP Kawaguchi-shi
- 优先权： JP2019-140852 20190731
- 国际申请： PCT/JP2020/029442 WO 20200731
- 主分类号： C12P19/34
- IPC分类号： C12P19/34 ; C07H19/10 ; C07H19/20
摘要：
A primer for amplifying a nucleic acid having a structure represented by the formula (1): where B represents a base, R1 represents a decomposable protecting group, and R2 represents hydrogen or a hydroxyl group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA, includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3′-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; and a photoirradiation unit for deprotecting R1 and forming a sticky end with a 3′-protruding end.
IPC结构图谱:
| C | 化学；冶金 |
| --C12 | 生物化学；啤酒；烈性酒；果汁酒；醋；微生物学；酶学；突变或遗传工程 |
| ----C12P | 发酵或使用酶的方法合成目标化合物或组合物或从外消旋混合物中分离旋光异构体 |
| ------C12P19/00 | 含有糖残基的化合物的制备 |
| --------C12P19/26 | .含氮碳水化合物的制备 |
| ----------C12P19/28 | ..N-葡萄糖苷 |
| ------------C12P19/30 | ...核苷酸 |
| --------------C12P19/34 | ....多核苷酸，如核酸、寡核糖核苷酸 |