Methods for preparing an animal hide for chrome tanning are provided. In particular, one or more embodiments include treating the hide with a first enzyme, a second enzyme, and a polysulfide. This treatment facilitates improved removal of hair from the hide. Furthermore, one or more embodiments include various combinations of treating the hide with a third enzyme, a liming agent, a pH-modifying agent, one or more sulfides, and one or more soaking agents. In addition, one or more embodiments include sanitizing the hide and liming the hide to further facilitate the processing of the animal hide into leather.