
基本信息:
- 专利标题: Cell Expansion
- 申请号:US16112498 申请日:2018-08-24
- 公开(公告)号:US20190062683A1 公开(公告)日:2019-02-28
- 发明人: Brian J. NANKERVIS , Mark E. JONES , Domicinda M. HILL
- 申请人: Terumo BCT, Inc.
- 申请人地址: US CO Lakewood
- 专利权人: Terumo BCT, Inc.
- 当前专利权人: Terumo BCT, Inc.
- 当前专利权人地址: US CO Lakewood
- 主分类号: C12M3/02
- IPC分类号: C12M3/02 ; C12M1/12 ; C12M1/00 ; C12N5/0783
摘要:
Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), helper, naïve, memory, or effector, for example.
公开/授权文献:
- US11624046B2 Cell expansion 公开/授权日:2023-04-11
IPC结构图谱:
C | 化学;冶金 |
--C12 | 生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程 |
----C12M | 酶学或微生物学装置 |
------C12M3/00 | 组织、人类、动物或植物细胞或病毒培养装置 |
--------C12M3/02 | .采用悬浮的方法 |