基本信息:
- 专利标题： METHOD FOR DETECTING GENETIC MUTATION
- 申请号：US15730013 申请日：2017-10-11
- 公开(公告)号：US20180037941A1 公开(公告)日：2018-02-08
- 发明人： Akio YAMANE , Ryoko IMAGAWA , Yuan YUAN
- 申请人： RIKEN GENESIS CO., LTD.
- 申请人地址： JP Tokyo
- 专利权人： RIKEN GENESIS CO., LTD.
- 当前专利权人： RIKEN GENESIS CO., LTD.
- 当前专利权人地址： JP Tokyo
- 优先权： JP2015-082495 20150414
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12N15/10
摘要：
A method for detecting a genetic mutation is provided. The method comprising: carrying out PCR using a template DNA, a forward primer, a reverse primer and a wild-type oligonucleotide; and detecting an amplified DNA. In the method, the wild-type oligonucleotide comprises LNA or BNA, and on a DNA strand of the template, with which the wild-type oligonucleotide is hybridized, a region with which the wild-type oligonucleotide is a hybridized and a region with which the forward primer or the reverse primer is hybridized partially overlap each other, or the regions are separated from each other by 1 to 18 bases.