A fabric sterilization tote apparatus and related methods of using and manufacturing the same is provided. The apparatus includes a flexible, bacteria-impenetrable material formed into a closable container, the closable container having a base, at least four sidewalls, and a cover, wherein an interior area of the closable container is defined by the base, the at least four sidewalls, and the cover. A bacteria-impenetrable closure is positioned between the cover and at least one of the four sidewalls, wherein the bacteria-impenetrable closure controls a sealing of the interior area of the closable container from an exterior atmosphere. In use, a medical instrument sterilization tray with the at least one medical instrument may be placed within the fabric sterilization tote apparatus after completion of a sterilization process.