基本信息:
- 专利标题： Endothelial-targeted Adenoviral Vectors, Methods and Uses Therefor
- 专利标题（中）：内皮靶向腺瘤病毒载体，其方法和用途
- 申请号：US14966078 申请日：2015-12-11
- 公开(公告)号：US20160145643A1 公开(公告)日：2016-05-26
- 发明人： Jefferey Arbeit , David Curiel
- 申请人： Washington University
- 申请人地址： US MO Saint Louis
- 专利权人： Washington University
- 当前专利权人： Washington University
- 当前专利权人地址： US MO Saint Louis
- 主分类号： C12N15/86
- IPC分类号： C12N15/86 ; C12N7/00 ; C07K14/715 ; A61K48/00 ; C12N9/78 ; C07K14/71
摘要：
Disclosed are adenovirus vectors comprising a ROBO4 enhancer/promoter operatively linked to a transgene. Also disclosed are adenovirus vectors comprising a chimeric AD5-T4 phage fibritin shaft, a trimerization domain displaying a myeloid cell-binding peptide (MBP), and a ROBO4 enhancer promoter operatively linked to a transgene. Also disclosed are methods of expressing a transgene in an endothelial cell in vivo, comprising administering to a mammal an adenovirus comprising a ROBO4 enhancer/promoter operatively linked to a transgene. Also disclosed are uses of the adenoviral vectors, including mobilization of granulocytes, monocytes and lymphocytes from bone marrow, mobilization of cancer cells in vivo, selective targeting of endothelial cells, and cancer treatment methods.
摘要（中）：
公开了包含可操作地连接到转基因的ROBO4增强子/启动子的腺病毒载体。 还公开了包含嵌合AD5-T4噬菌体纤维蛋白轴，显示骨髓细胞结合肽（MBP）的三聚体结构域和可操作地连接到转基因的ROBO4增强子启动子的腺病毒载体。 还公开了在体内在内皮细胞中表达转基因的方法，包括向哺乳动物施用包含可操作地连接到转基因的ROBO4增强子/启动子的腺病毒。 还公开了腺病毒载体的使用，包括从骨髓中移动粒细胞，单核细胞和淋巴细胞，体内移动癌细胞，选择性靶向内皮细胞和癌症治疗方法。