![POLYNUCLEOTIDE ENRICHMENT USING CRISPR-CAS SYSTEMS](/abs-image/US/2016/01/21/US20160017396A1/abs.jpg.150x150.jpg)
基本信息:
- 专利标题: POLYNUCLEOTIDE ENRICHMENT USING CRISPR-CAS SYSTEMS
- 专利标题(中):使用CRISPR-CAS系统的多核苷酸增殖
- 申请号:US14804068 申请日:2015-07-20
- 公开(公告)号:US20160017396A1 公开(公告)日:2016-01-21
- 发明人: Gordon M. Cann , Jeffrey G. Mandell , Alex Aravanis , Steven Norberg , Dmitry K. Pokholok , Frank J. Steemers , Farnaz Absalan , Leila Bazargan
- 申请人: ILLUMINA, INC.
- 申请人地址: US CA San Diego
- 专利权人: ILLUMINA, INC.
- 当前专利权人: ILLUMINA, INC.
- 当前专利权人地址: US CA San Diego
- 主分类号: C12P19/34
- IPC分类号: C12P19/34
摘要:
A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.
摘要(中):
一种富含靶核酸的方法,包括提供具有crRNA或其衍生物的内切核酸酶系统和Cas蛋白质或其变体。 所述crRNA或其衍生物含有与靶核酸区域基本上互补的靶特异性核苷酸区域; 使靶核酸与内切核酸酶系统接触以形成复合物; 并分离复合物,从而富集靶核酸。
公开/授权文献:
- US10457969B2 Polynucleotide enrichment using CRISPR-Cas systems 公开/授权日:2019-10-29
IPC结构图谱:
C | 化学;冶金 |
--C12 | 生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程 |
----C12P | 发酵或使用酶的方法合成目标化合物或组合物或从外消旋混合物中分离旋光异构体 |
------C12P19/00 | 含有糖残基的化合物的制备 |
--------C12P19/26 | .含氮碳水化合物的制备 |
----------C12P19/28 | ..N-葡萄糖苷 |
------------C12P19/30 | ...核苷酸 |
--------------C12P19/34 | ....多核苷酸,如核酸、寡核糖核苷酸 |