
基本信息:
- 专利标题: PRIMER SET FOR DETECTING BOVINE LEUKEMIA VIRUS AND USE THEREOF
- 专利标题(中):用于检测牛血清白蛋白病毒的初步设置及其使用
- 申请号:US14420720 申请日:2013-08-13
- 公开(公告)号:US20150203926A1 公开(公告)日:2015-07-23
- 发明人: Yoko Aida , Shin-nosuke Takeshima , Mayuko Tsunoda , Mikio Kawahara , Susumu Saito , Yuri Muramatsu , Yuan Yuan
- 申请人: RIKEN , RIKEN GENESIS CO., LTD.
- 优先权: JP2012-179972 20120814
- 国际申请: PCT/JP2013/071883 WO 20130813
- 主分类号: C12Q1/70
- IPC分类号: C12Q1/70
摘要:
A forward primer of a primer set in accordance with the present invention for detecting BLV is a mixture of (1) a first primer consisting of a polynucleotide including 15 or more successive bases including the 16th C in the base sequence of SEQ ID NO: 1 and (2) a second primer consisting of a plurality of kinds of polynucleotides including at least the first to 15th bases in the base sequence of SEQ ID NO: 2. Two or more of M, N, Y, K, and D which are included in the second primer are each a degenerate base which specifies two or more kinds of bases, and the second primer includes at least 10 kinds of polynucleotides including at least the first to 15th bases in the base sequences of SEQ ID NOs: 3 to 12.
摘要(中):
根据本发明的用于检测BLV的引物组的正向引物是(1)由包含15个或更多个连续碱基的多核苷酸组成的第一引物的混合物,包括SEQ ID NO:1的碱基序列中的第16位 和(2)由SEQ ID NO:2的碱基序列中至少第一至第十五个碱基的多种多核苷酸组成的第二引物.M,N,Y,K和D中的两个或多个是 包括在第二引物中的每一个都是指定两种或更多种碱基的简并碱基,第二引物包含至少10种在SEQ ID NO:3至12的碱基序列中至少包含第一至第十五碱基的多核苷酸 。