基本信息:
- 专利标题： PROMOTER-REGULATED DIFFERENTIATION-DEPENDENT SELF-DELETING CASSETTE
- 专利标题（中）：促销调节差异依赖自删除CASSETTE
- 申请号：US14571515 申请日：2014-12-16
- 公开(公告)号：US20150099296A1 公开(公告)日：2015-04-09
- 发明人： David Frendewey , Guochun Gong , Ka-Man Venus Lai , David M. Valenzuela
- 申请人： REGENERON PHARMACEUTICALS, INC.
- 主分类号： C12N15/85
- IPC分类号： C12N15/85
摘要：
Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of the recombinase in an differentiated cell but not an undifferentiated cell. Promoters include Blimp1, Prm1, Gata6, Gata4, Igf2, Lhx2, Lhx5, and Pax3. Targeting constructs with a cassette flanked on both sides by recombinase sites can be removed using a recombinase gene operably linked to a 3′-UTR that comprises a recognition site for an miRNA that is transcribed in undifferentiated cells but not in differentiated cells. The constructs may be included in targeting vectors, and can be used to automatically modify or excise a selection cassette from an ES cell, a non-human embryo, or a non-human animal.
摘要（中）：
提供靶向构建体和使用它们的方法用于细胞和非人动物中核酸序列的分化依赖性修饰。 提供了包含与重组酶可操作地连接的启动子的靶向构建体，其中所述启动子驱动分化细胞中但不是未分化细胞的重组酶的转录。 启动子包括Blimp1，Prm1，Gata6，Gata4，Igf2，Lhx2，Lhx5和Pax3。 可以使用可操作地连接到3'-UTR的重组酶基因来除去具有通过重组酶位点两侧的盒的靶向构建体，其包含在未分化细胞中但不在分化细胞中转录的miRNA的识别位点。 构建体可以包含在靶向载体中，并且可以用于自动修饰或从ES细胞，非人胚胎或非人动物中切除选择盒。