发明申请
US20120046339A1 Novel chorismate mutase gene from the potato cyst nematode globodera rostochiensis
有权
基本信息:
- 专利标题： Novel chorismate mutase gene from the potato cyst nematode globodera rostochiensis
- 专利标题（中）：来自马铃薯孢囊线虫的新型绒毛霉变种酶基因
- 申请号：US12460813 申请日：2009-07-24
- 公开(公告)号：US20120046339A1 公开(公告)日：2012-02-23
- 发明人： Xiaohong Wang , Shunwen Lu
- 申请人： Xiaohong Wang , Shunwen Lu
- 主分类号： A01N57/16
- IPC分类号： A01N57/16 ; C12Q1/68 ; A01P5/00 ; C12N15/82 ; C12N5/10 ; C12N15/113 ; C07H21/04 ; C07H21/02
摘要：
The nucleotide sequence of a 992 bp region of cDNA and the nucleotide sequence of a 1973 bp (or a 1913 bp) of genomic DNA of the Gr-cm-1 gene were determined for G. rostochiensis. PCR primers and probes specific for G. rostochiensis and G. pallida were generated. PCR assays, including a real-time TaqMan PCR were used to identify G. rostochiensis and G. pallida and to differentiate G. rostochiensis from G. pallida. Transgenic hairy roots expressing Gr-cm-1 dsRNA were generated. There was a 52% reduction in the average number of females per root in the Gr-cm-1 dsRNA transgenic lines when compared with the infected control lines.
摘要（中）：
对于罗氏假单胞菌确定了cDNA的992bp区域的核苷酸序列和Gr-cm-1基因的基因组DNA的1973bp（或1913bp）的核苷酸序列。 产生针对罗氏假单胞菌和G.pallida特异性的PCR引物和探针。 使用PCR实验，包括实时TaqMan PCR来鉴定罗氏假单胞菌（G. rostochiensis）和G.pallida，并分离G.pallida的G. rostochiensis。 产生表达Gr-cm-1 dsRNA的转基因毛根。 与感染的对照品系相比，Gr-cm-1 dsRNA转基因品系的每根根系平均数量减少了52％。