发明申请
US20110236983A1 SINGLE MOLECULE DETECTION AND SEQUENCING USING FLUORESCENCE LIFETIME IMAGING
审中-公开
基本信息:
- 专利标题： SINGLE MOLECULE DETECTION AND SEQUENCING USING FLUORESCENCE LIFETIME IMAGING
- 专利标题（中）：使用荧光生物成像的单分子检测和测序
- 申请号：US12980817 申请日：2010-12-29
- 公开(公告)号：US20110236983A1 公开(公告)日：2011-09-29
- 发明人： Joseph Beechem , Theofilos Kotseroglou , William Michael Lafferty , Mark F. Oldham
- 申请人： Joseph Beechem , Theofilos Kotseroglou , William Michael Lafferty , Mark F. Oldham
- 主分类号： G01N33/50
- IPC分类号： G01N33/50 ; G01N21/64 ; B82Y99/00
摘要：
A nucleic acid detection system and method are provided, in which excitation energy is transmitted from a pulsed excitation source to a reaction site including a fluorescence resonance energy transfer (FRET)-based dye system to generate a fluorescent signal at the reaction site, the fluorescent signal is detected by a detector from the reaction site, and detection of the fluorescent signal is respectively blocked and permitted at the detector by a detector gate this is timed based on an emission start time of the transmitted excitation energy.
摘要（中）：
提供了一种核酸检测系统和方法，其中激发能量从脉冲激发源传输到包括基于荧光共振能量转移（FRET）的染料系统的反应位点，以在反应位点产生荧光信号，荧光 信号被来自反应部位的检测器检测，并且荧光信号的检测分别被检测器门阻断并在检测器处允许，其基于发射的激发能量的发射开始时间来定时。