A detecting device for preventing a substrate from being scratched by an apparatus and a detecting method using the same is provided. The device comprises a detecting substrate, a controlling module, a transmitting module and a monitoring module. The controlling module detects a position information of a contact point between the apparatus and the detecting substrate and transmits the position information to the transmitting module. The transmitting module transmits the position information received from the controlling module to the monitoring module. The monitoring module determines the coordinates of the contact point between the apparatus and the detecting substrate according to the position information. The detecting substrate can be employed to simulate the substrate used in the real manufacture process of the TFT-LCD so that the apparatus in each step can be detected before the real manufacture process is performed. Thus, the apparatus can be adjusted before the real manufacture process is performed, and therefore the substrate can be effectively prevented from being scratched by the apparatuses in the real manufacture process.