基本信息:
- 专利标题： RNASE H-BASED ASSAYS UTILIZING MODIFIED RNA MONOMERS
- 专利标题（中）：使用修饰的RNA单体的RNASE H基测定
- 申请号：US12433896 申请日：2009-04-30
- 公开(公告)号：US20090325169A1 公开(公告)日：2009-12-31
- 发明人： Joseph Alan Walder , Mark Aaron Behlke , Scott Rose , Joseph Dobosy
- 申请人： Joseph Alan Walder , Mark Aaron Behlke , Scott Rose , Joseph Dobosy
- 申请人地址： US IA Coralville
- 专利权人： Integrated DNA Technologies, Inc.
- 当前专利权人： Integrated DNA Technologies, Inc.
- 当前专利权人地址： US IA Coralville
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34 ; C07H21/02 ; C07H21/04
摘要：
The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3′ end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.
摘要（中）：
本发明涉及用于各种生物测定的新型寡核苷酸化合物，例如核酸扩增，连接和测序反应。 该寡核苷酸在寡核苷酸的3'末端或其附近包含核糖核酸结构域和封闭基团。 这些化合物提供了先前未见的特异性增加水平。 还提供了进行核酸扩增，连接和测序的方法。 此外，本文还公开了含有寡核苷酸的试剂盒。