发明申请
US20090017490A1 DNA Fragment Method for Producing Transformant for Protein Production and Utilization Thereof
有权
基本信息:
- 专利标题： DNA Fragment Method for Producing Transformant for Protein Production and Utilization Thereof
- 专利标题（中）：用于产生蛋白质生产和利用的转化体的DNA片段方法
- 申请号：US10574112 申请日：2004-10-01
- 公开(公告)号：US20090017490A1 公开(公告)日：2009-01-15
- 发明人： Masashi Mori , Koji Dohi , Masayuki Ishikawa , Tetsuo Meshi , Masaki Nishikiori , Atsushi Tamai
- 申请人： Masashi Mori , Koji Dohi , Masayuki Ishikawa , Tetsuo Meshi , Masaki Nishikiori , Atsushi Tamai
- 优先权： JP2003-343747 20031001; JP2003-350091 20031008; JP2004-056912 20040301
- 国际申请： PCT/JP04/14487 WO 20041001
- 主分类号： C12Q1/04
- IPC分类号： C12Q1/04 ; C07H21/04 ; C12N15/63 ; C12P21/00 ; C12N5/10
摘要：
An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein gene of ToMV having a suppressor against a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cells, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.
摘要（中）：
通过转移重组番茄花叶病毒（ToMV）cDNA构建表达载体，其中具有抗病毒抗性反应的抑制剂的ToMV的外壳蛋白基因已被GFP基因取代成能够诱导类固醇的启动子的下游 激素依赖性转录。 在通过将上述表达载体转移到烟草BY-2细胞获得的转化烟草BY-2细胞中，诱导类固醇激素依赖性转录，从而能够扩增GFP基因的mRNA并诱导GFP表达。