发明申请
US20070128609A1 Ddr2 protein with activated kinase activity and preparation method thereof
审中-公开
基本信息:
- 专利标题： Ddr2 protein with activated kinase activity and preparation method thereof
- 专利标题（中）：具有活化激酶活性的Ddr2蛋白及其制备方法
- 申请号：US10595585 申请日：2004-11-01
- 公开(公告)号：US20070128609A1 公开(公告)日：2007-06-07
- 发明人： Beom-Seok Yang , Sung-Dae Park
- 申请人： Beom-Seok Yang , Sung-Dae Park
- 申请人地址： KR SEOUL Republic of Korea 137-041
- 专利权人： KOREA INSTITUE OF SCIENCE AND TECHNOLOGY
- 当前专利权人： KOREA INSTITUE OF SCIENCE AND TECHNOLOGY
- 当前专利权人地址： KR SEOUL Republic of Korea 137-041
- 优先权： KR10-2003-0076967 20031031
- 国际申请： PCT/KR04/02784 WO 20041101
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C07H21/04 ; C12P21/06 ; C12N9/12 ; C12N5/06 ; C12N15/86
摘要：
The present invention relates to a protein containing a modified DDR (Discoidin Domain Receptor) 2 cytosolic tyrosine kinase domain having an increased autophosphorylation and tyrosine kinase activity; a method for preparing a large amount of a protein containing DDR2 cytosolic tyrosine kinase domain, having an increased autophosphorylation and tyrosine kinase activity by inducing phosphorylations of tyrosines by a co-expression with Src or Src related proteins in host cells, or by H2O2 processing of host cells, or a site directed mutation modifying at least one of tyrosines to other amino acid; and a use thereof as a target material in developing medical drugs for treating a disease caused by an excessively activated DDR2 autophosphorylation and tyrosine kinase activity.
摘要（中）：
本发明涉及含有增加的自磷酸化和酪氨酸激酶活性的修饰的DDR（盘带蛋白结构域受体）2胞质酪氨酸激酶结构域的蛋白质; 通过与宿主细胞中的Src或Src相关蛋白的共表达，或通过H 2 O 2处理，通过诱导酪氨酸的磷酸化，具有增加的自磷酸化和酪氨酸激酶活性的DDR2胞质酪氨酸激酶结构域的蛋白质的制备方法 宿主细胞或将至少一种酪氨酸修饰至其它氨基酸的位点定向突变; 以及其用作开发用于治疗由过度活化的DDR2自磷酸化和酪氨酸激酶活性引起的疾病的药物的目标材料。