发明申请
US20060147949A1 PNA chip using plastic substrate coated with epoxy group-containing polymer, method of manufacturing the PNA chip, and method of detecting single nucleotide polymorphism using the PNA chip
失效
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基本信息:
- 专利标题: PNA chip using plastic substrate coated with epoxy group-containing polymer, method of manufacturing the PNA chip, and method of detecting single nucleotide polymorphism using the PNA chip
- 专利标题(中):使用涂有含环氧基聚合物的塑料基材的PNA芯片,PNA芯片的制造方法以及使用PNA芯片检测单核苷酸多态性的方法
- 申请号:US11156653 申请日:2005-06-21
- 公开(公告)号:US20060147949A1 公开(公告)日:2006-07-06
- 发明人: Jeongmin Ha , Jae Jang , In Kim
- 申请人: Jeongmin Ha , Jae Jang , In Kim
- 优先权: KR10-2004-0047677 20040624
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C08G63/91 ; C12M1/34 ; C08G63/48
摘要:
Provided is a PNA (Peptide Nucleic Acid) chip in which a probe PNA containing a desired DNA sequence is immobilized on a plastic substrate coated with an epoxy group-containing polymer. Therefore, single-stranded PNAs can be immobilized on a transparent plastic substrate by means of an epoxy group-containing polymer layer in an efficient and cost-effective manner. Fluorescence signal detection based on PNA/DNA hybridization enables identification of SNP (Single Nucleotide Polymorphism).
摘要(中):
提供了将含有所需DNA序列的探针PNA固定在涂布有含环氧基的聚合物的塑料基材上的PNA(肽核酸)芯片。 因此,可以通过含有环氧基的聚合物层以有效和成本有效的方式将单链PNA固定在透明塑料基材上。 基于PNA / DNA杂交的荧光信号检测能够鉴定SNP(单核苷酸多态性)。