基本信息:
- 专利标题： Bioprocess for the generation of cells from spheroid-forming cells
- 专利标题（中）：用于从球形细胞产生细胞的生物过程
- 申请号：US10189642 申请日：2002-07-03
- 公开(公告)号：US20030119107A1 公开(公告)日：2003-06-26
- 发明人： Stephen Dang , Peter W. Zandstra
- 优先权： CA2351156 20010704
- 主分类号： C12P021/06
- IPC分类号： C12P021/06
摘要：
The present inventors identified aggregation of embryonic stem cells and embryoid bodies (EBs) as the cause of the difficulty in generating large numbers of the embryonic stem cells (ES) cell-derived tissues. To counter this, the invention provides a novel bioprocess where aggregation of spheroid forming cells, such as embryonic stem cells and spheroids, such as EBs is controlled, such as by encapsulation of within a matrix. As a result, EBs can be generated with high efficiency and cultured in high cell density, well-mixed systems. Well-mixed conditions facilitate measurement and control of the bulk media conditions and allow for the use of scalable bioreactor systems for clinical production of tissue. Therefore, the invention enables generation of ES cell-derived tissue on a clinical scale. The invention is also applicable to any spheroid-forming cells and other types of pluripotent cells.
摘要（中）：
本发明人鉴定了胚胎干细胞和胚状体（EB）的聚集作为难以产生大量胚胎干细胞（ES）细胞衍生组织的原因。 为了解决这个问题，本发明提供了一种新颖的生物过程，其中例如通过在基质内封装来控制球形细胞如胚胎干细胞和球体如EB的聚集。 结果，可以高效率地生成EB，并在高细胞密度，良好混合的系统中培养。 良好混合的条件有助于测量和控制大量介质条件，并允许使用可扩展的生物反应器系统来临时生产组织。 因此，本发明能够在临床规模上产生ES细胞衍生的组织。 本发明也适用于任何球形细胞和其他类型的多能细胞。