发明申请
US20030036084A1 Nucleic acid detection method employing oligonucleotide probes affixed to particles and related compositions
审中-公开
基本信息:
- 专利标题： Nucleic acid detection method employing oligonucleotide probes affixed to particles and related compositions
- 专利标题（中）：使用固定在颗粒和相关组合物上的寡核苷酸探针的核酸检测方法
- 申请号：US10200723 申请日：2002-07-22
- 公开(公告)号：US20030036084A1 公开(公告)日：2003-02-20
- 发明人： Brian Hauser , Joerg Baier , Radoje T. Drmanac
- 主分类号： C12Q001/68
- IPC分类号： C12Q001/68 ; C12P019/34
摘要：
The invention relates to oligonucleotide probes attached to discrete particles wherein the particles can be grouped into a plurality of sets based on a physical property. A different probe is attached to the discrete particles of each set, and the identity of the probe is determined by identifying the discrete particles from their physical property. The physical property includes any that can be used to differentiate the discrete particles, and includes, for example, relative or absolute location, size, flourescence, radioactivity, electromagnetic charge, or absorbance, or label(s) may be attached to the particle such as a dye, a radionuclide, or an EML. The invention also relates to methods using the probes complexed with the discrete particles to analyze target nucleic acids.
摘要（中）：
本发明涉及附着于离散颗粒的寡核苷酸探针，其中基于物理性质将颗粒分成多组。 不同的探针连接到每组的离散颗粒，并且通过从其物理性质识别离散粒子来确定探针的身份。 物理性质包括可用于区分离散颗粒的任何物质，并且包括例如相对或绝对位置，大小，荧光，放射性，电磁电荷或吸光度，或标记可附着于颗粒，例如 作为染料，放射性核素或EML。 本发明还涉及使用与离散颗粒复合的探针来分析靶核酸的方法。