The present invention is used for testing cardiac efficacy and toxicity of drugs, and a cardiac chip that mimics a living body at the endomyocardial level including a culture solution supply unit for supplying a culture solution to cardiomyocytes and a cardiomyocyte supply unit including fixed cardiomyocytes. , A method for evaluating the efficacy of a drug on cardiomyocytes using the cardiac chip, and a method for evaluating the toxicity of a drug on cardiomyocytes using the cardiac chip. The use of an endomyocardial mimic cardiac chip for evaluating the efficacy and toxicity of a drug provided in the present invention can overcome inaccuracies due to differences between heterogeneity in animal experiments in cardiac research, and cardiomyocytes alone or cardiomyocytes It can be used as a platform for more accurate efficacy and toxicity evaluation of drugs under conditions similar to in vivo conditions by using hepatocytes and hepatocytes, and for microenvironment studies in the heart and other organ-on-a-chips. Since it can be applied to research, etc., it can be used to develop a human-on-a-chip that can effectively analyze drug efficacy and toxicity.