基本信息:
- 专利标题： 신규 미분화 줄기세포 제거 및 심근 순화 정제 배지
- 专利标题（英）：New undifferentiated stem cell removal and myocardial purification and refinement culture medium
- 专利标题（中）：新的未分化干细胞清除和心肌接种净化培养基
- 申请号：KR1020177002462 申请日：2015-07-16
- 公开(公告)号：KR1020170031152A 公开(公告)日：2017-03-20
- 发明人： 후쿠다게이이치 , 후지타준 , 도야마슈고
- 申请人： 하트 시드 가부시키가이샤
- 申请人地址： *-**-*, Jingumae, Shibuya-ku, Tokyo, Japan
- 专利权人： 하트 시드 가부시키가이샤
- 当前专利权人： 하트 시드 가부시키가이샤
- 当前专利权人地址： *-**-*, Jingumae, Shibuya-ku, Tokyo, Japan
- 代理人： 유미특허법인
- 优先权： JPJP-P-2014-146283 2014-07-16
- 国际申请： PCT/JP2015/071048 2015-07-16
- 国际公布： WO2016010165 2016-01-21
- 主分类号： C12N5/00
- IPC分类号： C12N5/00 ; C12N5/077 ; C12Q1/04
In the present invention, non-cardiac cells, or it can be more fully induce cell death relative to undifferentiated stem cells, and also to find a new condition, can be selected only the cardiomyocytes to the task. In order to solve this problem, in this matter, filed, as undifferentiated stem cells, cell culture media is used to cause cell death in, providing that it does not contain glutamine in the amino acid composition, and incubated in a cell culture medium with it, non-cardiac method of inducing cell death in cells also provided. In this matter, it filed Furthermore, as a cell culture medium that is used to selectively selected as the cardiac cells, the addition of lactic acid, pyruvic acid or acids, providing it does not include a sugar, which does not include the glutamine in the amino acid composition and the same time in cell culture, by culturing a mixture of cardiomyocytes and non-cardiomyocytes, methods for selectively selecting the cardiomyocytes also provided.
公开/授权文献:
- KR102241348B1 신규 미분화 줄기세포 제거 및 심근 순화 정제 배지 公开/授权日：2021-04-15