The present invention was prepared in the tissue can take biomaterial composed mainly of sodium hyaluronate, with a plurality of cross-linking reaction to increase the stability and durability of the product, by carrying out a plurality of precipitation, rehydration and cleaning process and the sterilization body It relates to the manufacturing method of taking tissue which can significantly improve the quality by completely removing the harmful components biomaterial, a first step of preparing a solution of sodium hydroxide; A second step of stirring a mixture of hyaluronic acid sodium predetermined amount of the above prepared sodium hydroxide solution; A third step of stirring by a cross-linking agent BDDE was added to the mixed solution prepared in the second step; A fourth step of the crosslinking reaction the sodium hyaluronate gel raw material generated by the third step; The fourth step to remove the cross-linking reaction of hyaluronic acid and ethanol precipitated by addition of ethanol to the sodium-gel raw material using, re solidifying the hyaluronic acid gel precipitated into the PBS solution, again with the fifth step; A sixth step of the precipitation and the rehydration sodium hyaluronate gel material through the fifth step over a predetermined three PBS washing solution for the; Then a solution of a predetermined PBS solution to sodium hyaluronate gel raw material complete cleaning over the sixth step at a predetermined ratio, to give the to give pulverized remove bubbles seventh step; The eighth step of filling the seventh dose may sodium hyaluronate gel tissue is completed through the manufacturing process biomaterial whether pre-filled syringe; And a ninth step of the first number of tissues sodium hyaluronate gel produced by the process taking 8 disinfecting biological material is if the pre-filled syringe filled autoclaved.