基本信息:
- 专利标题： 표적 핵산 검출을 위한 방법 및 조성물
- 专利标题（英）：Methods and compositions for detecting target nucleic acids
- 专利标题（中）：用于检测目标核酸的方法和组合物
- 申请号：KR1020137033544 申请日：2012-05-17
- 公开(公告)号：KR1020140064735A 公开(公告)日：2014-05-28
- 发明人： 테르브루겐로버트 , 리우옌보우 , 칠더스존레이 , 김창희 , 아베디마지드알.
- 申请人： 디엑스테리티 다이아그노스틱스 인코포레이티드
- 申请人地址： ***** S. Rancho Way, Suite ***, Rancho Dominguez, CA *****, U.S.A.
- 专利权人： 디엑스테리티 다이아그노스틱스 인코포레이티드
- 当前专利权人： 디엑스테리티 다이아그노스틱스 인코포레이티드
- 当前专利权人地址： ***** S. Rancho Way, Suite ***, Rancho Dominguez, CA *****, U.S.A.
- 代理人： 특허법인태평양
- 优先权： US61/486,817 2011-05-17
- 国际申请： PCT/US2012/038436 2012-05-17
- 国际公布： WO2012158967 2012-11-22
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
The present invention provides compositions, apparatus and methods for detecting one or more nucleic acid target present in the sample. The method of the present invention reversibly binding to the target nucleic acid that approaches close to each other and comprising the step of using two or more ligation probes possessing a complementary reactive moiety ligation. When such a probe is to be bonded to the target in the proper orientation, they may perform spontaneous chemical ligation reaction which generates a ligation product, and this is the ligation product is detected directly, or amplified to generate the amplicons is detected thereafter. The present invention also provides a method to prevent the degradation by stabilizing the RNA samples not have a significant effect on the analysis results.
公开/授权文献:
- KR102085242B1 표적 핵산 검출을 위한 방법 및 조성물 公开/授权日：2020-03-05