The present invention relates to the use and compositions comprising the compounds, and such compounds can be obtained by the breast milk oligosaccharides (HMO), or a precursor thereof in a variety-up methods, the above-described way. The method comprising a) optionally sialylated and / or Foucault misfire of lactose derivatives and salts thereof represented by the general formula 2 (wherein, R is a group removable by hydrogenolysis, R ₁ is independently Foucault chamber or H to each other, R ₄ are independently H or sialyl each other, if provided alone, or a compound represented by the general formula 2 is not a glycoside of lactose R-) optionally sialylated and / or lactose derivatives represented by the general formula 4 and Foucault misfire a salt thereof (and wherein, R ₁ is independently a Foucault chamber or H to each other, R ₄ is independently a sialyl or H to each other, if a single service, the compounds of general formula 4 is not lactose) of the formula (ⅰ) Lactobacillus -N- tetrahydro agarose (LNT): the formula (ⅱ) of Lactobacillus -N- tetrahydro agarose (LNT) derivatives (wherein, R is a group removable by hydrogenolysis): formula (ⅲ) of Lactobacillus -N- Neo-tetrahydro agarose (LNnT): and formula (ⅳ) -N- Lactobacillus Neo the Tet At least one provided in accordance with the b) step a): Lao (LNnT) derivative comprising the steps of: providing at least one compound or mixture of compounds selected from the group consisting of: (wherein, R is removed the possible group by hydrogenolysis) of the compound or the step of adding at least one enzyme containing trans glycosidase activity on a mixture of said compounds; And c) step b) comprises the step of incubating the mixture obtained in accordance with the.