基本信息:
- 专利标题: 복합 매트릭스로부터의 핵산 추출
- 专利标题(英):Nucleic acid extraction from complex matrices
- 专利标题(中):核酸从复杂矩阵中提取
- 申请号:KR1020127024050 申请日:2011-02-16
- 公开(公告)号:KR1020120138766A 公开(公告)日:2012-12-26
- 发明人: 아이센하워,크리스탈,알. , 로얄,브라이언,브이. , 엔구옌,린,엔.케이.
- 申请人: 사이시스 다이애그노스틱스, 인크.
- 申请人地址: *** Dale Avenue, Charlottesville, VA *****, U.S.A.
- 专利权人: 사이시스 다이애그노스틱스, 인크.
- 当前专利权人: 사이시스 다이애그노스틱스, 인크.
- 当前专利权人地址: *** Dale Avenue, Charlottesville, VA *****, U.S.A.
- 代理人: 황이남
- 优先权: US61/304,913 2010-02-16
- 国际申请: PCT/US2011/025051 2011-02-16
- 国际公布: WO2011103163 2011-08-25
- 主分类号: C12N15/10
- IPC分类号: C12N15/10 ; C12Q1/68
The invention from a composite matrix, such as a stool sample and the water sample on the adsorbent and the preferred protocol for extracting a nucleic acid such as DNA and RNA. The adsorbent is an activated carbon coated with a material such as polyvinyl pyrrolidone, dextran, and coconut powder. The adsorbent may be used in micro-Century fuse spin column, the adsorbent may be present as a slurry in a storage solution in a micro spin-column Sentry fuse. The sample can be prepared by the steps comprising: through a centrifugation step, adding a protease in the supernatant, and micro-Century fuse spin column containing a coated active carbon to pass the supernatant liquid is mixed to generate an eddy current in buffer solution is. The main components comprising a buffer, a protease and spin columns can be packaged as a kit.