基本信息:
- 专利标题： 양전하를 띤 탄소나노튜브를 사용한 핵산 추출방법
- 专利标题（英）：Method for extracting nucleic acid using carbon nanotube charged electropositive
- 专利标题（中）：使用碳纳米管填充电解质提取核酸的方法
- 申请号：KR1020110025042 申请日：2011-03-21
- 公开(公告)号：KR1020120107543A 公开(公告)日：2012-10-04
- 发明人： 박수민 , 신원상 , 김해원 , 서재원 , 전세계 , 장원철
- 申请人： 박수민 , 단국대학교 산학협력단
- 申请人地址： 충청북도 청주시 흥덕구 풍년로***번길 **, *층호 (가경동)
- 专利权人： 박수민,단국대학교 산학협력단
- 当前专利权人： 박수민,단국대학교 산학협력단
- 当前专利权人地址： 충청북도 청주시 흥덕구 풍년로***번길 **, *층호 (가경동)
- 代理人： 이종혁
- 主分类号： C12N15/10
- IPC分类号： C12N15/10 ; C12Q1/68 ; G01N33/50
摘要：
PURPOSE: A method for isolating nucleic acids using carbon nanotubes with a positive charge is provided to enhance a binding force with nucleic acids and to improve nucleic acid yield. CONSTITUTION: A method for isolating nucleic acids using carbon nanotubes with a positive charge comprises: a step of mixing a sample containing the nucleic acids with the carbon nanotubes(S100); a step of adding washing buffer to the mixture and washing(S200); and a step of adding a DNA elution reagent to the mixture and collecting the nucleic acids(S300). The method further comprises a step of modifying a non-polar carbon nanotube into a carbon nanotube with positive charge. [Reference numerals] (S100) Reaction step of sample and carbon nanotube; (S200) Washing step by adding washing buffer to mixture; (S300) Collection step by adding DNA elution reagent and collecting nucleic acids
摘要（中）：
目的：提供使用具有正电荷的碳纳米管分离核酸的方法以增强与核酸的结合力并提高核酸产率。 构成：使用具有正电荷的碳纳米管分离核酸的方法包括：将含有核酸的样品与碳纳米管混合的步骤（S100）; 向混合物中加入洗涤缓冲液并进行洗涤的步骤（S200）; 以及向混合物中加入DNA洗脱剂并收集核酸的步骤（S300）。 该方法还包括将非极性碳纳米管修正为带正电荷的碳纳米管的步骤。 （附图标记）（S100）样品和碳纳米管的反应步骤; （S200）通过向混合物中加入洗涤缓冲液进行洗涤步骤; （S300）收集步骤，加入DNA洗脱剂和收集核酸
公开/授权文献:
- KR101329221B1 양전하를 띤 탄소나노튜브를 사용한 핵산 추출방법 公开/授权日：2013-12-31