基本信息:
- 专利标题: 캔디다 알비칸스의 검출용 핵산 탐침
- 专利标题(英):Nucleic acid probes for detection of candida albicans
- 专利标题(中):用于检测坎迪达阿尔卑斯山羊的核酸探针
- 申请号:KR1020060111598 申请日:2006-11-13
- 公开(公告)号:KR1020060123688A 公开(公告)日:2006-12-04
- 发明人: 이상엽 , 유승민 , 유소영 , 장경희 , 금기창 , 유내춘 , 유원민 , 이진 , 김준명
- 申请人: 메디제네스(주) , 유원민 , 금기창 , 유내춘
- 申请人地址: 서울특별시 금천구 디지털로 ***, ***호 (가산동, 남성프라자)
- 专利权人: 메디제네스(주),유원민,금기창,유내춘
- 当前专利权人: 메디제네스(주),유원민,금기창,유내춘
- 当前专利权人地址: 서울특별시 금천구 디지털로 ***, ***호 (가산동, 남성프라자)
- 代理人: 이처영
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
A nucleic acid probe for detection of Candida albicans is provided to detect and identify Candida albicans as a non-viral infectious microorganism in a biological sample through the hybridization with rRNA of Candida albicans. The nucleic acid probe for detection of Candida albicans contains the isolated nucleic acid molecule comprising the nucleotide sequence selected from SEQ ID NO:398(TCAAGAACGAAAGTT, C. alic 006), SEQ ID NO:399(AAGGATTGACAGATT, C. alic 007) and SEQ ID NO:400(CATTAATCAAGAACG, C. alic 008), wherein the nucleic acid probe is derived from rRNA gene of Candida albicans. A composition comprises the nucleic acid probe for detection of Candida albicans containing the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:398, SEQ ID NO:399 or SEQ ID NO:400. A method for detecting and identifying Candida albicans in a biological sample comprises the steps of: (a) isolating and concentrating a polynucleic acid in the biological sample; (b) amplifying the polynucleic acid with appropriate sense and anti-sense primer pair; (c) hybridizing the amplified polynucleic acid with the nucleic acid probe for detection of Aeromonas hydrophila containing the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:398, SEQ ID NO:399 or SEQ ID NO:400; (d) detecting the hybrids; and (e) identifying the strains in the biological sample from the different hybridization signals.
摘要(中):
提供了用于检测白色念珠菌的核酸探针,以通过与白色念珠菌的rRNA杂交来检测并鉴定生物样品中的非病毒感染性微生物的白色念珠菌。 用于检测白色假丝酵母的核酸探针含有分离的核酸分子,其包含选自SEQ ID NO:398(TCAAGAACGAAAGTT,C.alic 006),SEQ ID NO:399(AAGGATTGACAGATT,C.alic 007)和SEQ ID NO:399 SEQ ID NO:400(CATTAATCAAGAACG,C.alic 008),其中所述核酸探针源自白色念珠菌的rRNA基因。 组合物包含用于检测含有包含SEQ ID NO:398,SEQ ID NO:399或SEQ ID NO:400的核苷酸序列的核酸分子的白色念珠菌的核酸探针。 用于检测和鉴定生物样品中的白色念珠菌的方法包括以下步骤:(a)分离并浓缩生物样品中的多核酸; (b)用适当的有义和反义引物对扩增多核酸; (c)将扩增的多核酸与用于检测含有包含SEQ ID NO:398,SEQ ID NO:399或SEQ ID NO:400的核苷酸序列的核酸分子的嗜水气单胞菌的核酸探针杂交; (d)检测杂种; 和(e)从不同杂交信号鉴定生物样品中的菌株。
公开/授权文献:
- KR100781559B1 캔디다 알비칸스의 검출용 핵산 탐침 公开/授权日:2007-12-03