基本信息:
- 专利标题: 인간 로타바이러스의 활성을 저해하는 항체의 생산용 항원및 그의 제조방법과 이용
- 专利标题(英):An antigen for production of an antibody against human rotavirus, and a preparing method and a use thereof
- 专利标题(中):用于生产人类脊髓灰质炎抗生素抑制活性的抗原及其生产及其使用
- 申请号:KR1020010059731 申请日:2001-09-26
- 公开(公告)号:KR1020030026654A 公开(公告)日:2003-04-03
- 发明人: 이석준 , 김희주 , 윤순대 , 김순옥 , 김경수 , 김완주
- 申请人: 주식회사 씨트리
- 申请人地址: 경기도 남양주시 경강로 ** (일패동)
- 专利权人: 주식회사 씨트리
- 当前专利权人: 주식회사 씨트리
- 当前专利权人地址: 경기도 남양주시 경강로 ** (일패동)
- 代理人: 김윤배
- 主分类号: A61K39/15
- IPC分类号: A61K39/15
摘要:
PURPOSE: A process of mass-preparing a specific antigen used for production of an antibody which inhibits the activity of human rotavirus causing diarrhea is provided. The obtained eggs from birds immunized with the antigen have prevention and treatment effects on diarrhea. A functional food containing the egg yolk of the eggs has preventive and therapeutic effects on diarrhea in an infant and a young child. CONSTITUTION: The mass producing method of the specific antigen comprises the step of: selectively separating a gene encoding VP7 antigen by performing RT PCR using, as a template, genomic DNA of human rotavirus; extracting vector DNA by inserting the separated gene into a suitable vector, transforming a suitable strain with the vector and amplifying the vector; amplifying the gene encoding the antigen protein by performing PCR using, as a template, the extracted vector DNA; cloning the amplified gene into a suitable vector and transforming a suitable host with the vector; and culturing the host, followed by overexpressing the antigen.
摘要(中):
目的:提供一种大规模制备用于制备抑制引起腹泻的人轮状病毒活性的抗体的特异性抗原的方法。 用抗原免疫的鸟获得的鸡蛋具有对腹泻的预防和治疗作用。 含有鸡蛋蛋白的功能性食物对婴儿和幼儿的腹泻具有预防和治疗作用。 构成:特定抗原的批量生产方法包括以下步骤:使用人轮状病毒的基因组DNA作为模板,通过进行RT PCR选择性分离编码VP7抗原的基因; 通过将分离的基因插入合适的载体中来提取载体DNA,用载体转化合适的菌株并扩增载体; 通过使用提取的载体DNA作为模板进行PCR来扩增编码抗原蛋白的基因; 将扩增的基因克隆到合适的载体中并用载体转化合适的宿主; 并培养宿主,然后过表达抗原。
信息查询:
EspacenetIPC结构图谱:
A | 人类生活必需 |
--A61 | 医学或兽医学;卫生学 |
----A61K | 医用、牙科用或梳妆用的配制品 |
------A61K39/00 | 含有抗原或抗体的医药配制品 |
--------A61K39/12 | .病毒性抗原 |
----------A61K39/15 | ..呼肠病毒科,如小牛腹泻病毒 |