基本信息:
- 专利标题: 후혼성 표지화 및 범용 코드화에 의한 핵산 검출 및 정량화
- 专利标题(英):KR101932628B1 - Nucleic acid detection and quantification by post-hybridization labeling and universal encoding
- 申请号:KR1020137000372 申请日:2011-06-07
- 公开(公告)号:KR101932628B1 公开(公告)日:2018-12-27
- 发明人: 프레지본,다니엘,씨. , 스토너,이삭 , 윈드무쓰,안드레아스 , 에릅스,티모시
- 申请人: 파이어플라이 바이오웍스, 인코포레이티드
- 申请人地址: One Kendall Square, Building ****W, *rd Floor, Cambridge, MA *****, U.S.A.
- 专利权人: 파이어플라이 바이오웍스, 인코포레이티드
- 当前专利权人: 파이어플라이 바이오웍스, 인코포레이티드
- 当前专利权人地址: One Kendall Square, Building ****W, *rd Floor, Cambridge, MA *****, U.S.A.
- 代理人: 특허법인 남앤남
- 优先权: US61/352,018 2010-06-07; US61/365,738 2010-07-19; US61/387,958 2010-09-29
- 国际申请: PCT/US2011/039531 2011-06-07
- 国际公布: WO2011156434 2011-12-15
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12N15/11 ; G01N33/53
The present invention, first of all, the post-provides methods and compositions for the detection and quantification of target nucleic acid with a labeled hybrid. In some embodiments, the invention provides a method for detecting the presence and / or water (abundance) of a target nucleic acid in the sample, which is (a) binding to the capture sequence and the universal adapters, each combined with the target nucleic acid contact phase of the plurality of nucleic acid probe and a sample consisting of a neighboring adapter sequence; (B) in the condition that there are one or more of the universal adapter and the individual target nucleic acid and separate universal adapter, both may be combined in the same individual a nucleic acid probe, the step of culturing a plurality of the probe and the sample; (C) step is a separate universal adapter to carry out the reaction in combination with a separate target nucleic acid and wherein the nucleic acid probe hybridizes to the same individual; (D) a step of detecting the presence of a plurality of one or more general purpose associated with the nucleic acid probe adapter, thereby detecting the presence of target nucleic acid in the sample along. Typically, the universal adapters are detectable substance or other detection trigger is labeled with labeling groups. In some embodiments, the plurality of nucleic acid probes suitable for the present invention are attached to a substrate or object (e.g., a microarray, or particles).
公开/授权文献:
- KR1020130082495A 후혼성 표지화 및 범용 코드화에 의한 핵산 검출 및 정량화 公开/授权日:2013-07-19