The invention together generate antimicrobial nanoparticles of a different polarity and improve the microbial collection performance by uniformly attaching antibacterial particles antimicrobial composite nanostructures generated with the box by bonding it to create a antimicrobial composite nanostructures to filter media and filters relates to anti-bacterial filter media manufacturing apparatus and method with also inhibit the proliferation microorganisms on the filter medium with antibiotic complex nanostructures that can be sustained for a long time the antibacterial activity on microorganisms, the antimicrobial filter using an antibacterial compound nanostructure according to the present invention filter material production apparatus includes a first generation first antimicrobial nanoparticles to generate antimicrobial nanoparticles device, and a second charged with polarity second generated second antimicrobial nanoparticles to generate antimicrobial nanoparticles device and electrically charged to a first polarity by combining the first and second antimicrobial nanoparticles antimicrobial nanoparticles through human antimicrobial composite nano Comprised, including the antibiotic complex nanostructure binding chamber for producing a crude product, wherein the first antimicrobial nanoparticles generation apparatus, the first antimicrobial nanoparticles production chamber and the first antibacterial nano-charges the first antimicrobial solution in a first polar It is converted into particles of the first first electrostatic spray nozzle, the first first anti-bacterial solution supply unit and the first electrostatic supplying a first antimicrobial solution into the electrostatic spray nozzle for commanded the inner space of the antimicrobial nanoparticles production chamber and is configured to include a first high voltage generator for applying a high voltage of a first polarity to the spray nozzle, wherein the second antimicrobial nanoparticles recovery apparatus of the second antimicrobial nanoparticles production chamber, a charge the second antimicrobial solution in a second polarity the first claim for supplying a second antibiotic in the solution is converted into two antimicrobial nanoparticles second electrostatic spray nozzle and the second nozzle for an electrostatic spray commanded in the inner space of the second antimicrobial nanoparticles production chamber 2 It characterized in that the bacteria comprise a solution supply unit and a second high voltage generator for applying a high voltage of a second polarity to the second electrostatic spray nozzle.