基本信息:
- 专利标题： 헤테로랍디티스 메기디스의 액체 배양방법
- 专利标题（英）：Liquid mass culture method of heterorhabditis megidis
- 专利标题（中）：液体质量培养方法MEGIDIS
- 申请号：KR1020120079196 申请日：2012-07-20
- 公开(公告)号：KR101262733B1 公开(公告)日：2013-05-09
- 发明人： 김태완 , 김애정 , 김효현 , 장상일 , 남욱호
- 申请人： ㈜엠알이노베이션
- 申请人地址： 대구광역시 북구 대학로 **, **층(산격동,경북대학교테크노파크아이티융합산업빌딩)
- 专利权人： ㈜엠알이노베이션
- 当前专利权人： ㈜엠알이노베이션
- 当前专利权人地址： 대구광역시 북구 대학로 **, **층(산격동,경북대학교테크노파크아이티융합산업빌딩)
- 代理人： 안경주
- 主分类号： C12N5/07
- IPC分类号： C12N5/07 ; C12N5/02
摘要：
PURPOSE: A liquid culturing method of heterohabditis megidis is provided to manufacture a large amount of liquid with a sufficient concentration by forming an optimum environment for cultivation. CONSTITUTION: A liquid culturing method of heterohabditis megidis is for culturing insect pathogenic eelworms. The liquid culturing method of heterohabditis megidis transfers oxygen to a culture medium by using a microsparger. The liquid culturing method of heterohabditis megidis inoculate the insect pathogenic eelworms to the culture medium by 3,000-4,500/mL and transfers the culture medium by using the microsparger. The insect pathogenic eelworms are cultivated by inoculating the insect pathogenic eelworms in the culture medium by using seed-cultured eelworms by using a nutrient medium. The concentration of the final insect pathogenic eelworms is 200-400×10^3/mL.
摘要（中）：
目的：通过形成培养的最佳环境，提供了大量异构多糖的液体培养方法，以制备足够浓度的大量液体。 构成：大肠杆菌的液体培养方法是用于培养昆虫致病性鳗虫。 异构体megidis的液体培养方法通过使用微量滴定器将氧气转移到培养基中。 异源性多糖的液体培养方法将昆虫致病性鳗虫以3,000-4,500 / mL的速度接种培养基，并通过使用微量滴定器转移培养基。 通过使用营养培养基使用种子培养的蚯蚓，在培养基中接种昆虫致病性鳗虫来培养昆虫致病性鳗虫。 最终的昆虫致病性鳗鱼的浓度为200-400×10 ^ 3 / mL。