基本信息:
- 专利标题： 염색체내 형광단백질 유전자 일부가 삽입된 세포를포함하는 이분자 형광 상보 시스템 및 이를 이용한 이분자형광 상보 기법
- 专利标题（英）：System of Biomolecular Fluorescence Complementation Containing a Cell Inserted a Part of Fluorescence Protein Gene In Chromosome and Methods of Biomolecular Fluorescence Complementation By Using It
- 专利标题（中）：生物分子荧光互补系统在染色体中插入一部分荧光蛋白基因的细胞和使用它的生物分子荧光互补方法
- 申请号：KR1020060096709 申请日：2006-09-30
- 公开(公告)号：KR100825290B1 公开(公告)日：2008-04-25
- 发明人： 허원기 , 성민경
- 申请人： 재단법인서울대학교산학협력재단
- 申请人地址： 서울특별시 관악구 관악로 * (신림동)
- 专利权人： 재단법인서울대학교산학협력재단
- 当前专利权人： 재단법인서울대학교산학협력재단
- 当前专利权人地址： 서울특별시 관악구 관악로 * (신림동)
- 代理人： 윤여강; 이문섭; 양부현
- 主分类号： C12N15/08
- IPC分类号： C12N15/08 ; C12N5/12
효모, 이분자 형광 상보 기법, 형광단백질, 단백질 상호작용, 세포내 위치
The present invention relates to a plasmid vector for applying the complementary bimolecular fluorescence (bimolecular fluorescence complementation) for real-time analysis of intracellular protein interactions, and this technique. Unlike the invention of the bimolecular fluorescence complementary technique is conventional extracellular way analysis or protein interaction analysis with the artificial protein expression, interaction occurs between the proteins expressed in their natural state, from their own promoter in the cell in real time, in addition to analysis has the advantage of being able to analyze up to my position occurring cell-protein interactions. Yeast, bimolecular fluorescence complementary techniques, fluorescent protein, protein interactions, cell location