基本信息:
- 专利标题： スフェロイドの作製方法及び該方法に用いる培地
- 专利标题（英）：JPWO2016143808A1 - Medium used for making and method of spheroids
- 申请号：JP2017505367 申请日：2016-03-09
- 公开(公告)号：JPWO2016143808A1 公开(公告)日：2018-02-15
- 发明人： 山田 勝也 , 小野 幸輝
- 申请人： 国立大学法人弘前大学
- 申请人地址： 青森県弘前市文京町1番地
- 专利权人： 国立大学法人弘前大学
- 当前专利权人： 国立大学法人弘前大学
- 当前专利权人地址： 青森県弘前市文京町1番地
- 代理人： 正林 真之; 新山 雄一
- 优先权： JP2015046420 2015-03-09
- 主分类号： C12N5/02
- IPC分类号： C12N5/02
The present invention is a simple method, and an object thereof is to provide a method for producing a high-quality spheroid in large quantities. The present invention is also a growth factor and attachment factors or without using extracellular matrix or special culture system, it aims to provide a method for easily producing a spheroid. The present invention, a method for manufacturing a spheroid, characterized in that a glucose-containing medium glucose for culturing cells using the culture medium consists essentially L- glucose was provided. Further, growth factors, adhesion factors, and extracellular matrix in a medium without the addition, methods of making the spheroids are provided in a culture system that does not use a three-dimensional support.
公开/授权文献:
- JP6721868B2 スフェロイドの作製方法及び該方法に用いる培地 公开/授权日：2020-07-15