基本信息:
- 专利标题： ペプチド断片の調製方法およびそれに用いられるペプチド断片調製用キット、ならびに分析方法
- 专利标题（英）：Preparation and peptide fragments prepared for the kit is used to that of the peptide fragments, as well as methods of analysis
- 专利标题（中）：制备和肽片段制备的套盒为此使用的肽片段，并且分析方法
- 申请号：JP2015535281 申请日：2013-09-09
- 公开(公告)号：JPWO2015033479A1 公开(公告)日：2017-03-02
- 发明人： 崇史 嶋田 , 典子 深尾 , 智景 青木 , 孝明 佐藤
- 申请人： 株式会社島津製作所
- 申请人地址： 京都府京都市中京区西ノ京桑原町1番地
- 专利权人： 株式会社島津製作所
- 当前专利权人： 株式会社島津製作所
- 当前专利权人地址： 京都府京都市中京区西ノ京桑原町1番地
- 代理人： 新宅 将人; 吉本 力
- 优先权： JP2013074292 2013-09-09
- 主分类号： G01N33/68
- IPC分类号： G01N33/68 ; C12P21/06 ; G01N27/62 ; G01N33/50
The present invention uses a protease, the protein was cut to a method of preparing a peptide fragment.
The method of the present invention, immobilized porous substrate proteins to be cut (25) within the pores (29) and (20), and protease (15) is fixed to the surface particles (10) the comprising the step of contacting in a liquid. In the present invention, the average particle size of the fine particles (10) is larger than the average pore diameter of the porous body (20). According to the method of the present invention, it regioselectively cleaving antibodies substrate proteins (25).
The peptide fragments obtained by the above method, by analyzing by mass spectrometry or the like may perform detection and quantitative analysis of proteins in the antibody.
公开/授权文献:
- JP5924455B2 ペプチド断片の調製方法およびそれに用いられるペプチド断片調製用キット、ならびに分析方法 公开/授权日：2016-05-25