基本信息:
- 专利标题： The method of removing the mismatch binding polynucleotide
- 申请号：JP2003339714 申请日：2003-09-30
- 公开(公告)号：JP4342890B2 公开(公告)日：2009-10-14
- 发明人： 徹 土谷
- 申请人： 富士フイルム株式会社
- 专利权人： 富士フイルム株式会社
- 当前专利权人： 富士フイルム株式会社
- 优先权： JP2003339714 2003-09-30
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12P19/34 ; C12Q1/68
摘要：
A labeled polynucleotide is subjected to hybridization with oligonucleotide probes fixed respectively to regions on a supporting material. With a restriction enzyme, a single-stranded moiety of the labeled polynucleotide having failed to form a double strand with each probe is separated from a double strand having been formed by the labeled polynucleotide and each probe. Thereafter, an electrode is located at a surface of each region on the supporting material, and a voltage is applied to the electrode. The labeled polynucleotide, which has undergone a mismatch binding with a certain oligonucleotide probe, is thus separated from the corresponding region on the supporting material.
公开/授权文献:
- JP2005102580A Method for removing mismatch-bound polynucleotide 公开/授权日：2005-04-21