Disclosed is a method for producing in using surface-modified Fluorescent dye containing resin particle liquid as stain, a fluorescent dye encapsulated resin particle liquid dispersion of the bright points after dyeing is less than conventional products I will provide a.
A (was coupled first affinity molecule, a certain amount of the signal values ​​at the same number of fluorescent dye alone which is included in a fluorescent dye encapsulated resin particles contained in the solution) × (a fluorescent dye encapsulated the value of the resin particles one surface affinity number of molecules bound per) × {(the emission intensity of the fluorescent dye containing resin particles) / (emission intensity of a dye alone which is included in a fluorescent dye encapsulated resin particles)} is 0.5 or more, step selects the one is 1.2 or less, and after the selection process, the signal sum of the surface area of ​​the fluorescent dye containing resin particles contained in the fluorescent dye encapsulated resin particle solution for (T) (S the ratio of the values ​​of) (S / T) was diluted to a predetermined value, comprising the step of adjusting the concentration of the fluorescent dye containing resin particle liquid, the method of producing a fluorescent dye encapsulated resin particle solution.
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