The present invention,
A method for coupling two molecules by reverse electron request Diels-Alder reaction involving (DARinv), the method comprising the following reactions (a) and (b).
(A) as a diene component,
-COOR
_-C (O) NR 2
_-CX 3 (X is a halogen)
- Halogen -CN
_-SO 2 -R or _{SO 3 -R}
-PR 2 _(wherein, R, H, an alkyl group, an aryl group, a heterocyclic group, which alkyl group optionally, OH groups, SH groups, halogens, aryl groups, heterocyclic groups, nitro group, carboxamido group, may be substituted with an amino group.)
- Heterocycle [the heterocyclic ring has at least one carboxyl group is substituted with a sulfonic acid group or a phosphonic group, and 1, 2 or 3 N atoms, O or S atoms, the ring size Saga 5-membered ring or a 6-membered ring]
Having on the ring one or more electron-withdrawing group selected from triazines as diene component, or tetrazine.
(B) as a dienophile component, (hetero) Isolated olefins in isolated double or triple bond, or optionally straight-chain or branched-chain optionally containing a heteroatom chain hydrocarbon chains optionally in carbocycle sexual double or triple bond.